Antibiotic resistance frequently causes current treatment options to become ineffective against many bacteria. Thus, utilising gold complexes as antibacterial agents has been proposed. Nevertheless, these complexes such as gold(I) thiolates and their antibacterial effects are not well studied. Therefore, this study was undertaken to evaluate the antibacterial activity of phosphanegold(I) thiolate compounds with the general structure Ph3PAu[SC(OR)=NC6H5] with different R-groups of CH3 (AAu1), CH2CH3 (AAu2), and CH(CH3)2 (AAu3) as well as the synergistic effects between these compounds and chloramphenicol antibiotic against methicillin-resistant Staphylococcus aureus (MRSA). Broth dilution assay was conducted to determine the minimum inhibitory concentration (MIC) of the tested compounds against MRSA. Then, the drug combination effects were investigated through a checkerboard assay using fractional inhibitory concentration (FIC) index calculations. Results showed that phosphanegold(I) thiolate compounds were potent against MRSA, where AAu1 and AAu3 (MIC=3.125µg/mL) had better antibacterial activities than AAu2 (MIC=6.25µg/mL). This suggested the variation in R-groups of AAu1-3 compounds may influence their antibacterial efficacy. However, the AAu1-3 compounds and chloramphenicol drug combinations manifested indifferent interactions (FICI=1.25-1.50). Notably, this study has demonstrated the undisguised bactericidal effects exhibited by phosphanegold(I) thiolate compounds against MRSA. Thus, the combination of these compounds with other antibiotics as a novel treatment for antibiotic-resistance bacterial infections can be further considered.