Mazen Emad Mohamed Wardany

Figure#1: (Amoebicidal Assay) Compounds effect on cell count of A. castellanii Trophozoites.

This Assay was performed to evaluate the potential of the compounds to kill or inhibit the growth of the amoeba. 

As shown in Figure 1 Amoebicidal Assay, Fluconazole has slightly more effect on the A. castellanii Trophozoites than Fluconazole loaded with graphene oxide.

Figure#2: (Amoebistatic Assay) Compounds effect on cell count of A. castellanii Trophozoites.

This Assay was performed to evaluate the potential of the compounds to slow down or stop the growth of the amoeba. 

As shown in Figure 2 for Amoebistatic Assay, Fluconazole loaded with graphene oxide has slightly more effect than Fluconazole in eradicating A. castellanii Trophozoites.

Figure#3: (Excystation Assay) Compounds effect on cell count of A. castellanii Trophozoites.

This Assay was performed to evaluate the potential of the compounds to eliminate or prevent the excystation process (Cyst to Trophozoite) of the amoeba. 

As shown in Figure 3 for Excystation Assay, Fluconazole loaded with graphene oxide has slightly more effect than Fluconazole in eradicating A. castellanii Trophozoites.

Figure#4: (Encystation Assay) Compounds effect on cell count of A. castellanii Cysts.

This Assay was performed to evaluate the potential of the compounds to eliminate or prevent the encystation process (Trophozoite to Cyst) of the amoeba.

As shown in  Figure 4 for Encystation Assay, Fluconazole loaded with graphene oxide has slightly more effect than Fluconazole in eradicating A. castellanii Cysts.

Figure#5: Calculated cell viability % of the Compounds on human HaCaT cells.

This Assay was performed to measure the metabolic activity of cells, which can be used as an indicator of cell viability, proliferation, and cytotoxicity. It was used to evaluate the effects of the compounds on the human HaCaT cells. 

In Figure 5 for MTT Assay, Fluconazole loaded with graphene oxide has proven to be drastically less toxic towards HaCaT cells in comparison with Fluconazole. The Cell Viability % of Fluconazole is 27.36% while Fluconazole loaded with graphene oxide remains ahead of Fluconazole with a Cell Viability % of 99.36%. 

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