One of the most crucial points at which biochemistry and medicine interact is in the drug development process. Proteins serve as enzymes, receptors, and transporters, and drugs bind to these proteins to block or otherwise modify their functions. Therefore, it is essential for medication development to understand these molecules and the pathways through which they function.
Recombinant DNA technology has enabled the commercialization of several protein treatments throughout the past 20 years. The surge in the use of recombinantly synthesized drug-based proteins has led to raising concerns regarding their stability during long-term storage and effective administration methods without leading to any negative immunogenic adverse reactions.
The selection of a buffer for the formulation of a particular protein is a difficult task since the degree to which a certain protein may be stabilised or destabilised by utilising a buffer relies on several factors. The aim of this project is to examine the exact effects of pH buffers on protein stability using Raman spectroscopy.