Current Research

Current projects in my laboratory include:

Investigation of the utility of TDP43 and FUS as biomarkers of amyotrophic lateral sclerosis (ALS) and fronto-temporal lobar degeneration (FTLD) (funded by the Sir John Fisher foundation)

Characterisation of monoclonal antibodies raised against TDP43

Characterisation of the Caenharabditis elegans homolog of the C9ORF72 protein (implicated in ALS and FTLD)

Research Interests

Protein aggregation is a hallmark of many neurodegenerative disorders including Alzheimer’s Disease (AD), Huntington’s Disease (HD), Parkinson’s Disease (PD) and Motor Neurone Disease (MND). Each of these diseases is characterised by abnormal aggregation of one or more specific proteins. Typically familial forms of these diseases are associated with mutations in the gene encoding the protein with propensity to aggregate. For example familial forms of motor neurone disease may be associated with mutations in the genes encoding TDP-43 or FUS proteins, which aggregate abnormally in MND patients. Our work is currently focused on the a-synuclein protein (associated with PD), b-synuclein (associated with Dementia with Lewy Bodies, DLB) and the TDP43 and FUS proteins (associated with MND). Our work focuses on expressing in E. coli and purifying ‘normal’ wild type and ‘mutant’ disease associated forms of these proteins and characterising their aggregation propensities. We have raised antibodies to recombinant proteins and are currently characterising their specificities with a view to developing further biomarker assays.

Homologous recombination has an important role in facilitating cell survival following damage to DNA; defects in recombination repair mechanisms can lead to genomic instability and initiation of carcinogenesis. Our studies of homologous recombination focus on characterising recombination proteins from bacteria, flies, parasites and humans expressed in E. coli and purified by column chromatography. Our aim is to understand protein interactions required in different organisms to mediate recombination reactions. Activities of proteins consistent with a role in homologous recombination are assayed using a variety of techniques. This includes electrophoretic assays for DNA binding and strand exchange, thin layer chromatography assays for ATPase activity, and visualisation of protein-DNA complexes by transmission electron microscopy. In addition we are characterising interaction between recombination proteins by yeast two-hybrid, co-immunoprecipitation and bead-based assays.

Current Teaching

Currently I am Director of Studies for the IBMS accredited BSc (Hons) Biomedical Science degree, and for the 2nd and 3rd years of the BSc/MSci Biomedicine programmes.

I convene and teach on the following modules:

BIOL253 Genetics: Lectures and practicals on fundamental principles of DNA metabolism including DNA structure, replication reombination and repair. Lectures on gene functionand cancer genetics. Workshops on laboratory calculations and human genetic diseases.

BIOL281 Clinical Biochemistry - practicals on analytical methods in clinical biochemistry

BIOL282 Cellular Pathology

BIOL311 Genetics - practicals on recombinant protein expression and purification, lectures on diseases associated with defective DNA repair processes.

BIOL312 Medical Genetics - practicals on forensic DNA profiling, workshops on inherited human disorders, lectures on repeat expansion disorders

BIOL386 and BIOL387 project module convener

Career Details

Education

• BSc (Hons) Biochemistry, Hull University, 1982
• Ph.D. (Genetics) University of Nottingham, 1988

Previous academic positions

• 1987-1989 Postdoctoral research assistant, MRC Radiobiology Unit Harwell
• 1989-1992 Postdoctoral Research Fellow, Imperial Cancer Research Fund, South Mimms, Herts.
• 1992-1999 Chief Scientific Officer, Imperial Cancer Research Fund, South Mimms, Herts.